Journal: Hormones (Athens, Greece)

Article Title: Anticancer effects of metformin on neuroendocrine tumor cells in vitro.

doi: 10.14310/horm.2002.1517

Figure Lengend Snippet: Figure 3. Effect of metformin on PI3K/AKT/mTORC1 sig- naling in neuroendocrine. Human pancreatic BON1 (A, B), bronchopulmonary NCI-H727 (C, D) and midgut GOT1 (E, F) neuroendocrine tumor cells were treated with indicated concentrations of metformin for 24 and 48 hrs, respectively. Subsequently the expression of pAKT, AKT, pEBP1, EBP1, IGFR (A, C and E) as well as pP70S6K, P70S6K, pS6, S6 (B, D and F) and ą-actin loading control was evaluated by Western blot analysis. A representative blot out of 3 independently per- formed experiments is shown.

Article Snippet: After blocking in 2% non-fat dried milk, the membranes were incubated overnight in appropriate dilutions of antibodies against pAkt (Ser 473) (#4060), Akt (#2920), pERK (Thr202/Tyr204) 1/2 (#4370), pP70S6K (Thr389) (#9234), P70S6K (#9202), p4EBP1 (Ser65) (#9451), 4EBP1 (#9644), pS6 (Ser235/6 (#4858) and Ser240/4 (#5364)), S6 (#2317), pGSK3 (Ser21/9) (#9331), GSK3 (#9315), IGFR (#3027), EGFR (#4267), PARP (#9542) (all from Cell Signaling, Danvers, MA), Erk 1/2 (06-182; Millipore).

Techniques: Expressing, Control, Western Blot

Journal: Hormones (Athens, Greece)

Article Title: Anticancer effects of metformin on neuroendocrine tumor cells in vitro.

doi: 10.14310/horm.2002.1517

Figure Lengend Snippet: Figure 4. Effect of metformin and rapamycin on IGF1-induced signaling in neuroendocrine tumor cells. Human pancreatic BON1 (left panel) and bronchopulmonary NCI-H727 (right panel) neuroendocrine tumor cells were pretreated with indi- cated concentrations of metformin or rapamycin for 1 hr prior to induction with IGF1 for 24 hrs. The expression of pIGFR, IGFR, pAKT, AKT, pS6, S6 and ą-actin loading control was evaluated by Western blot analysis. A representative blot out of 3 independently performed experiments is shown.

Article Snippet: After blocking in 2% non-fat dried milk, the membranes were incubated overnight in appropriate dilutions of antibodies against pAkt (Ser 473) (#4060), Akt (#2920), pERK (Thr202/Tyr204) 1/2 (#4370), pP70S6K (Thr389) (#9234), P70S6K (#9202), p4EBP1 (Ser65) (#9451), 4EBP1 (#9644), pS6 (Ser235/6 (#4858) and Ser240/4 (#5364)), S6 (#2317), pGSK3 (Ser21/9) (#9331), GSK3 (#9315), IGFR (#3027), EGFR (#4267), PARP (#9542) (all from Cell Signaling, Danvers, MA), Erk 1/2 (06-182; Millipore).

Techniques: Expressing, Control, Western Blot

Journal: bioRxiv

Article Title: Development of the visual system in social poison frog tadpoles

doi: 10.1101/2022.10.18.512729

Figure Lengend Snippet: ( A ) Begging tadpoles had higher pS6 staining in the ganglion cell layer (GCL) and inner nuclear layer (INL) compared to non-begging tadpoles. ( B ) Photomicrographs showing pS6 staining in the GCL and INL, but not in the outer nuclear layer (ONL). Dotted lines approximately separate layers. Scale bar = 10 um. ( C ) The number of pS6-stained cells positively correlated to tadpole stage. ( D ) While metamorphic begging tadpoles had higher activation in the ganglion cell layer, there was no difference in pS6 staining in the retina of control, begging, and aggressive pre-metamorphic tadpoles. Different lowercase letters represent significant differences (P<0.05).

Article Snippet: Slides were allowed to come to room temperature, washed with 1x PBS 3×10min, blocked in 1x PBS with 5% normal goat serum (NGS) and 0.3% triton-X, incubated in 1:5000 rabbit anti-pS6 (Invitrogen; cat# 44-923G) prepared in blocking solution overnight at 4°C.

Techniques: Staining, Activation Assay